RRML - New high-throughput liquid chromatographic tandem mass spectrometry assay for therapeutic drug monitoring of carvedilol in children with congestive heart failure
AMLR

ISSN online: 2284-5623

ISSN-L: 1841-6624

Impact factor (2019): 0.945

Rejection rate (2020): 75%

Română English


Clarivate analytics (ISI) Impact factor


Advanced search


Top 10 downloaded articles
- November 2020 -
 
Molecular epidemiology and the... 13
Antibacterial and antitumor ac... 12
The Diagnostic Value of Monocy... 12
LSM3, NDUFB3, and PTGS2 may be... 11
Assessment of circulating tumo... 11
Romanian Review of Laboratory ... 9
The potential of programmed de... 9
Troubleshootings in RNA extrac... 7
Impact of preanalytical variab... 4
Validation of GOD / PAP method... 3

Log in

Concept, Design & Programming
Dr. Adrian Man

   
 
Nr. 15(2)/2009
XML
TXT

New high-throughput liquid chromatographic tandem mass spectrometry assay for therapeutic drug monitoring of carvedilol in children with congestive heart failure

Angela Butnariu, Daniela-Saveta Popa, Laurian Vlase, Mariana Andreica, Dana Muntean, Sorin Leucuţa


Abstract:

A new high-throughput liquid chromatography tandem mass spectrometry (LC-MS/MS) assay for the quantification of carvedilol in human plasma was developed and validated. The separation was performed on a Zorbax SB-C18 column under isocratic conditions using a 34:66 (v/v) mixture of acetonitrile and 0.2% (v/v) formic acid in water at 42 ºC with a flow rate of 0.3 mL/min. The detection of carvedilol was performed in multi-ple reaction monitoring (MRM) mode (m/z 222, 224, 283 from m/z 407). The human plasma samples (0.2 mL) were deproteinized with methanol, and aliquots of 15 μL from the supernatants obtained after centrifugation were directly injected into the chromatographic system. The method shows a good linearity (r > 0.9979), precision (CV < 12.9 %) and accuracy (bias < 7.8 %) over the range of 1.63-180.9 ng/mL plasma. The lower limit of quantification (LLOQ) was 1.63 ng/mL and the recovery was between 95-109.8 %. The method is not expensive, it needs a minimum time for plasma sample preparation and has a run-time of 2 min for instrument analysis (retention time of carvedilol was 1.75 min). The developed and validated high-throughput method is very simple, rapid and efficient, with wide applications in clinical therapeutic drug monitoring, pharmacokinetics and bioequivalence studies.

Keywords: carvedilol,LC-MS/MS,therapeutic drug monitoring

 
  PDF Download full text PDF
(103 KB)
     
 
How to cite
Butnariu A, Popa DS, Vlase L, Andreica M, Muntean D, Leucuţa S. New high-throughput liquid chromatographic tandem mass spectrometry assay for therapeutic drug monitoring of carvedilol in children with congestive heart failure. Rev Romana Med Lab. 2009;15(2):7-15