RRML - High Prevalence of CTX-M Group of Extended Spectrum Beta-Lactamases in Enterobacteriaceae strains isolated from the North-East of Romania
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Dr. Adrian Man
   
 
Nr. 19(3)/2011
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High Prevalence of CTX-M Group of Extended Spectrum Beta-Lactamases in Enterobacteriaceae strains isolated from the North-East of Romania

Cristina Tuchiluş, Ana Irina Mereuţă, Aida Bădescu, Dumitru Buiuc


Abstract:

The antibiotic resistance mediated by extended-spectrum beta-lactamases (ESBL) is a phenomenon that creates serious therapeutic problems around the world. The aim of this study was to detect the presence of CTX-M ESBLs enzymes in Enterobacteriaceae isolates from the North-East of Romania and the antimicrobial resistance profile of the CTX-M-producing strains. Material and methods. One hundred and twelve Escherichia coli and Klebsiella pneumoniae strains isolated from patients admitted to "Sf. Spiridon" Teaching Hospital from Iasi, between January-October 2009 were included. ESBL production was determined using a double-disc synergy test. The presence of CTX-M-type beta-lactamase genes was initially screened by PCR using universal primers for the conserved region of bla CTX-M genes. The PCR positive isolates were further tested using individuals pairs of primers to identify to which CTX-M group the gene belonged. Results. A total of 41 strains were positive for CTX-M genes: 39 strains (29 E. coli and 10 K. pneumoniae) carried CTX-M group 1 related genes, one strain of E. coli with CTX-M group 2 gene and another strain of E. coli with CTX-M group 9 gene. These enterobacteria strains demonstrated a high level of resistance to cephalosporins, with cefotaxime minimum inhibitory concentration (MIC) ≥ 4-fold greater than ceftazidime MIC. Conclusions. The study revealed that CTX-M-1 group is the dominant ESBL-type in both E. coli and K. pneumoniae tested strains. In addition, this is the first report of E. coli producing CTX-M -9 related ESBL in Romania.

Keywords: CTX-M,extended-spectrum beta-lactamases,enterobacteria

 
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How to cite
Tuchiluş C, Mereuţă AI, Bădescu A, Buiuc D. High Prevalence of CTX-M Group of Extended Spectrum Beta-Lactamases in Enterobacteriaceae strains isolated from the North-East of Romania. Rev Romana Med Lab. 2011;19(3):251-7