RRML - Emergence of rotavirus G9 in 2012, as the dominant genotype in Turkish children with diarrhea, in a university hospital in Ankara
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Nr. 27(2)/2019 DOI:10.2478/rrlm-2019-0021
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Research article

Emergence of rotavirus G9 in 2012, as the dominant genotype in Turkish children with diarrhea, in a university hospital in Ankara

Aylin Altay Koçak, Merve Aydın, Takashi Matsumoto, Takaaki Yahiro, Buket Dalgıç, Gulendam Bozdayi, Kamruddin Ahmed

Correspondence should be addressed to: Gulendam Bozdayi

Abstract:

Introduction: Rotavirus infection is a major cause of morbidity and mortality in infants and young children with diarrhea throughout the world. Material and Methods: In this study, we aimed to determine the detection rate of rotavirus infection in 181 children less than 5 years of age presenting with acute gastroenteritis and admitted to a tertiary care hospital in Ankara, Turkey, from April to November 2012. We documented the epidemiological data by elucidating the prevalent genotypes. Stool specimens were collected, and rotavirus antigen in the samples was detected using ELISA. G and P genotypes were determined by RT-PCR via type specific primers. The nucleotide sequence of the concerned genes was determined by Sanger sequencing and phylogenetic analysis was performed by neighbor-joining method. Results: Of the 181 samples, 28 (15.5%) were positive for the rotavirus antigen. Twenty-seven samples were positive for G genotypes and 21 were positive for P genotypes. Genotypes G1 (7.1%), G2 (7.1%), G3 (7.1%), G4 (3.6%), G9 (71.5%) and P4 (3.6%), P8 (71.4%) were identified. Genotype G9P[8] (50%) was predominant in the combination of G and P genotypes. Most of the G9 strains of this study formed an independent cluster in Lineage III, except two strains which clustered with an Ethiopian G9 strain of 2012. Conclusions: It seems that during 2012 season, genotype G9P[8] increased significantly in Ankara due to a new circulating strain of G9.

Keywords: children, genotype, rotavirus infection, Turkey

Received: 5.11.2018
Accepted: 7.3.2019
Published: 4.4.2019

 
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How to cite
Koçak AA, Aydın M, Matsumoto T, Yahiro T, Dalgıç B, Bozdayi G, et al. Emergence of rotavirus G9 in 2012, as the dominant genotype in Turkish children with diarrhea, in a university hospital in Ankara. Rev Romana Med Lab. 2019;27(2):209-18. DOI:10.2478/rrlm-2019-0021