RRML - Significance of graft quality in allogeneic hematopoietic stem cell transplantation
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Dr. Adrian Man

   
 
Nr. 16(3)/2009
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Significance of graft quality in allogeneic hematopoietic stem cell transplantation

Cristian Jinca, Smaranda Arghirescu, Andrada Oprişoni, Loredana Balint-Gib, Mihaela Baica, Margit Serban


Abstract:

Purpose of the study. The content of the graft plays a major role for the outcome in patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT). This retrospective study aims to assess the correlation between the graft content and chimerism on one hand and on the other hand to find the predictive role of the graft content on engraftment kinetics. Material and method. Sixteen grafts were analyzed before transplantation by flowcytometry and chimerism in terms of percent of donor cells was prospectively investigated at specific time points either by real-time polymerase chain reaction (PCR) or by fluorescence in situ hybridization (FISH). Correlations between the two parameters were assessed by using SPSS 8.0 for Windows. Results. There was no significant correlation between the CD34+ progenitor content of the graft and percent of donor cells at the investigated time points. Instead, our data shows that the T cell content of the graft correlated significantly with the percent of donor cells on days +30 and +90. The respective Pearson coefficients were r = 0.692 (p < 0.01) on day +30 and r = 0.575 (p < 0.05) on day +90. A significant correlation was also found between the CD34+ cell content and time to engraftment (r = - 0.767; p < 0.01). Discussion. Chimerism is a predictive parameter used to assess post-transplant disfunctions. The significant correlations of the T cell graft content with the chimerism can further predict outcome. The higher the number of CD34+ cells in the graft, the earlier the patient engrafts.

Keywords: Allogeneic hematopoietic stem cell transplantation,flowcytometry,chimerism,engraftment.

 
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How to cite
Jinca C, Arghirescu S, Oprişoni A, Balint-Gib L, Baica M, Serban M. Significance of graft quality in allogeneic hematopoietic stem cell transplantation. Rev Romana Med Lab. 2009;16(3):21-7