RRML - Tissue protection immunohistochemistry – an innovative method to improve diagnosis accuracy in prostate cancer

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Nr. 18(2)/2010

Tissue protection immunohistochemistry – an innovative method to improve diagnosis accuracy in prostate cancer

Denisa Anderco, Elena Lazăr, Sorina Tăban, Felix Mic, Angela Borda, Andrada Loghin, Alis Dema

Abstract:

Prostate biopsy is the standard procedure used in the diagnosis of prostate cancer. Immunostains, additional to standard stains, are sometimes necessary for correct interpretation and classification of atypical, minute lesions. However, immunohistochemistry becomes noncontributory in the situation of loss of suspected foci after repeated sections from the paraffin block. This situation, which is not at all infrequent in prostate pathology, led to the design of special techniques that make immunostaining possible. We further present the results of an innovative technique of tissue protection immunohistochemistry which allows the immunostaining on an initially H&E-stained slide. Unlike other methods described before, this technique does not require H&E destaining prior to session and did not involve saving intervening unstained sections for possible immunohistochemical examination. The great advantage of tissue protection immunohistochemistry is that it allows simultaneous examination of H&E and immunostaining on the same slide. It only requires that the original H&E-stained sections to be placed on charged slides.

Keywords: technique,prostate biopsy,diagnosis,immunohistochemistry

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Anderco D, Lazăr E, Tăban S, Mic F, Borda A, Loghin A, et al. Tissue protection immunohistochemistry – an innovative method to improve diagnosis accuracy in prostate cancer. Rev Romana Med Lab. 2010;18(2):63-9