RRML - The efficiency of sodC gene / N. meningitidis detection in comparison with the classical methods for the diagnosis of meningococcal infection
AMLR

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2020): 75%

Română English


Journal Metrics

Impact Factor 0.493
Five Year Impact Factor 0.531
SNIP 0.373
JCI 0.17


Advanced search


Top 10 downloaded articles
- December 2022 -
 
Downregulation of hsa-miR-4328... 5
Normality assessment, few para... 4
Setting up an own laboratory p... 3
The relationship between matri... 2
New Para-Clinical Investigatio... 2
New high-throughput liquid chr... 2
General Principles of Haematop... 2
Genetic risk factors for throm... 2
The importance of the new prog... 2
Detection of chromosomal imbal... 2

Log in

Concept, Design & Programming
Dr. Adrian Man

   
 
Nr. 23(1)/2015 DOI:10.1515/rrlm-2015-0008
XML
TXT

The efficiency of sodC gene / N. meningitidis detection in comparison with the classical methods for the diagnosis of meningococcal infection

Roxana Elena Nemescu, Ramona Gabriela Ursu, Carmen Mihaela Dorobăț, Luminița Smaranda Iancu


Abstract:

Meningococcal infection requires a fast and accurate diagnostic method in order to correctly initiate the antibiotic therapy. The aim of our study was to assess the efficiency of Real Time PCR –Taq Man using sod C gene / N. meningitidis in comparison with the classical methods for the diagnosis of meningococcal infection – direct microscopy, cultivation, latex agglutination and blood culture. We have detected 24/44 (54.54%) patients with meningococcal infection. In both cases of patients with / without previous antibiotic therapy before admission, the AUC (area under curve) had the highest values for RT PCR in CSF and blood analysis. This sod C RT-PCR assay is a highly sensitive and specific method for detection of Neisseria meningitis and it would be useful to include this method like a multiplex in routine testing of patients with clinical meningococcal infection for other etiological agents also.

Keywords: Neisseria meningitidis, meningococcal infection, Real-Time PCR, performance criteria

Received: 30.5.2014
Accepted: 26.2.2015
Published: 12.3.2015

 
  PDF Download full text PDF
(569 KB)
     
 
How to cite
Nemescu RE, Ursu RG, Dorobăț CM, Iancu LS. The efficiency of sodC gene / N. meningitidis detection in comparison with the classical methods for the diagnosis of meningococcal infection. Rev Romana Med Lab. 2015;23(1):21-30. DOI:10.1515/rrlm-2015-0008