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Research article Autoantibodies Against ROS-Human Serum Albumin-A Potent Immunological marker in Depressed Individuals with Smoking History Subuhi Sherwani, Mohd W. A. Khan
Correspondence should be addressed to: Mohd W. A. Khan
Abstract: Background: Depression is one of the significant problems in adults that accounts for up to five percent of cases worldwide. Methods: Volunteers were divided into eight groups, and their serum samples were tested for FBG, carbonyl contents, IFN-γ and TNF-α. Reactive oxygen species (ROS) modified human serum albumin (HSA) (ROS-HSA) was used as an antigen and levels of serum autoantibodies were estimated by direct binding and inhibition ELISA in all subjects. Results: Significant biophysical structural modifications were observed in ROS-HSA with increased carbonyl contents compared to native-HSA (N-HSA). Significantly high levels of carbonyl content (2.68 ± 0.33 nmol/mg protein; p > 0.001) and pro-inflammatory cytokines IFN-γ (7.4 ± 0.61 pg/ml; p > 0.001) and TNF-α (1.47 ± 0.23 pg/ml; p > 0.001) were detected in serum samples from F-D-S. Similarly, a high level of autoantibodies against ROS-HSA was observed in females who were depressed and smokers (F-D-S) group (0.89 ± 0.07; p > 0.001) compared to males who were both depressed and smokers (M-D-S) (0.66 ± 0.049). Furthermore, inhibition ELISA results exhibited high recognition of serum autoantibodies from F-D-S subjects (78.6 ± 5.7 mean maximum percentage inhibition MMPI) compared to M-D-S (58.8 ± 5.2 MMPI) subjects. Conclusion: Incoherence, long term unchecked chronic psychological stress may cause oxidation of blood proteins, which subsequently result in structural alterations of biomolecules, thus generating new-epitopes, capable of inducing autoantibodies specific for ROS-modified proteins. These autoantibodies may be a potential marker for subjects suffering from depression to understand the state of immune imbalance. Keywords: depression, proinflammatory cytokines, oxidative stress, autoantibodies, HSA
Received: 30.5.2022 |
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Sherwani S, Khan MWA. Autoantibodies Against ROS-Human Serum Albumin-A Potent Immunological marker in Depressed Individuals with Smoking History. Rev Romana Med Lab. 2022;30(4):399-411. DOI:10.2478/rrlm-2022-0039
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