RRML - Tissue protection immunohistochemistry – an innovative method to improve diagnosis accuracy in prostate cancer

Main page
News
RJLabM Credits
Archive
- Current issue
- Ahead of print
- List of published issues
- 2024
  - Nr.2/2024
  - Nr.1/2024
- 2023
  - Nr.4/2023
  - Nr.3/2023
  - Nr.2/2023
  - Nr.1/2023
  - Supplement 1/2023
- 2022
  - Nr.4/2022
  - Nr.3/2022
  - Nr.2/2022
  - Nr.1/2022
  - Supplement 1/2022
- 2021
  - Nr.4/2021
  - Nr.3/2021
  - Nr.2/2021
  - Nr.1/2021
- 2020
  - Nr.4/2020
  - Nr.3/2020
  - Nr.2/2020
  - Nr.1/2020
  - Supplement 1/2020
- 2019
  - Nr.4/2019
  - Nr.3/2019
  - Nr.2/2019
  - Nr.1/2019
  - Supplement 1/2019
- 2018
  - Nr.4/2018
  - Nr.3/2018
  - Nr.2/2018
  - Nr.1/2018
  - Supplement 1/2018
  - Supplement 2/2018
  - Supplement 3/2018
- 2017
  - Nr.4/2017
  - Nr.3/2017
  - Nr.2/2017
  - Nr.1/2017
  - Supplement 1/2017
- 2016
  - Nr.4/2016
  - Nr.3/2016
  - Nr.2/2016
  - Nr.1/2016
  - Supplement 1/2016
- 2015
  - Nr.4/2015
  - Nr.3/2015
  - Nr.2/2015
  - Nr.1/2015
  - Supplement 1/2015
- 2014
  - Nr.4/2014
  - Nr.3/2014
  - Nr.2/2014
  - Nr.1/2014
  - Supplement 1/2014
- 2013
  - Nr.4/2013
  - Nr.3/2013
  - Nr.2/2013
  - Nr.1/2013
  - Supplement 1/2013
- 2012
  - Nr.4/2012
  - Nr.3/2012
  - Nr.2/2012
  - Nr.1/2012
  - Supplement 1/2012
- 2011
  - Nr.4/2011
  - Nr.3/2011
  - Nr.2/2011
  - Nr.1/2011
  - Supplement 1/2011
- 2010
  - Nr.4/2010
  - Nr.3/2010
  - Nr.2/2010
  - Nr.1/2010
  - Supplement 1/2010
- 2009
  - Nr.4/2009
  - Nr.3/2009
  - Nr.2/2009
  - Nr.1/2009
  - Supplement 1/2009
- 2008
  - Nr.4/2008
  - Nr.3/2008
  - Nr.2/2008
  - Nr.1/2008
  - Supplement 1/2008
- 2007
  - Nr.4/2007
  - Nr.3/2007
  - Nr.2/2007
  - Nr.1/2007
- 2006
  - Nr.4/2006
  - Nr.3/2006
  - Nr.2/2006
  - Nr.1/2006
- 2005
  - Nr.1/2005
Instructions for authors
Online applications
- Send an article
- Online payments
Editorial
About us
Links

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2020): 75%

Journal Metrics

Impact Factor 0.5
Five Year Impact Factor 0.5
JCI 0.12

Top 10 downloaded articles - May 2024 -

A comprehensive review of Prof...	26
The role of nitro oxidative fa...	15
Effect of Systemic Inflammator...	15
Diagnostic value of stress myo...	14
Romanian Review of Laboratory ...	13
Evaluation of routine laborato...	13
Correlations of expressions of...	12
Monitoring the sensitivity of ...	11
Evaluation of cytotoxicity and...	10
Recomandarea comună EFLM-COLA...	9

Concept, Design & Programming
Dr. Adrian Man

Nr. 18(2)/2010

Tissue protection immunohistochemistry – an innovative method to improve diagnosis accuracy in prostate cancer

Denisa Anderco, Elena Lazăr, Sorina Tăban, Felix Mic, Angela Borda, Andrada Loghin, Alis Dema

Abstract:

Prostate biopsy is the standard procedure used in the diagnosis of prostate cancer. Immunostains, additional to standard stains, are sometimes necessary for correct interpretation and classification of atypical, minute lesions. However, immunohistochemistry becomes noncontributory in the situation of loss of suspected foci after repeated sections from the paraffin block. This situation, which is not at all infrequent in prostate pathology, led to the design of special techniques that make immunostaining possible. We further present the results of an innovative technique of tissue protection immunohistochemistry which allows the immunostaining on an initially H&E-stained slide. Unlike other methods described before, this technique does not require H&E destaining prior to session and did not involve saving intervening unstained sections for possible immunohistochemical examination. The great advantage of tissue protection immunohistochemistry is that it allows simultaneous examination of H&E and immunostaining on the same slide. It only requires that the original H&E-stained sections to be placed on charged slides.

Keywords: technique,prostate biopsy,diagnosis,immunohistochemistry

Download full text PDF
(425 KB)

Anderco D, Lazăr E, Tăban S, Mic F, Borda A, Loghin A, et al. Tissue protection immunohistochemistry – an innovative method to improve diagnosis accuracy in prostate cancer. Rev Romana Med Lab. 2010;18(2):63-9