RRML - Separation methods used for the study of proteome

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Dr. Adrian Man

Nr. 8(3)/2007

Separation methods used for the study of proteome

Ileana Funduc

Abstract:

The term “proteome” defines the total set of proteins expressed by its genome in a given cell, tissue or biological fluid, at a given time. The purpose of proteomics is the identification and quantification of proteins from the given biological material, analysis of the changes in protein expression in normal versus diseased cells, characterization of post-translational modifications, the study of protein – protein interactions as well as other applications. Proteomic analyses can be classified as three main categories: expression proteomics, bioinformatics analysis and functional proteomics. The study of proteome uses for the image analyses electrophoresis, chromatography and for the identification of protein sequences, especially mass spectroscopy. The different methods of electrophoresis and visualization of protein spots in gels are hereby described. If the level of the sensitivity and specificity of the proteomic patterns will have maximum values, the disease diagnosis using them will revolutionize medicine.

Keywords: electrophoresis,chromatography,mass spectrometry

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Funduc I. Separation methods used for the study of proteome. Rev Romana Med Lab. 2007;8(3):45-53