RRML - Development of the acid elution method (Kleihauer test) in the assessment of fetomaternal hemorrhage

Main page
News
RJLabM Credits
Archive
- Current issue
- Ahead of print
- List of published issues
- 2025
  - Nr.1/2025
  - Nr.2/2025
- 2024
  - Nr.4/2024
  - Nr.3/2024
  - Nr.2/2024
  - Nr.1/2024
  - Supplement 1/2024
- 2023
  - Nr.4/2023
  - Nr.3/2023
  - Nr.2/2023
  - Nr.1/2023
  - Supplement 1/2023
- 2022
  - Nr.4/2022
  - Nr.3/2022
  - Nr.2/2022
  - Nr.1/2022
  - Supplement 1/2022
- 2021
  - Nr.4/2021
  - Nr.3/2021
  - Nr.2/2021
  - Nr.1/2021
- 2020
  - Nr.4/2020
  - Nr.3/2020
  - Nr.2/2020
  - Nr.1/2020
  - Supplement 1/2020
- 2019
  - Nr.4/2019
  - Nr.3/2019
  - Nr.2/2019
  - Nr.1/2019
  - Supplement 1/2019
- 2018
  - Nr.4/2018
  - Nr.3/2018
  - Nr.2/2018
  - Nr.1/2018
  - Supplement 1/2018
  - Supplement 2/2018
  - Supplement 3/2018
- 2017
  - Nr.4/2017
  - Nr.3/2017
  - Nr.2/2017
  - Nr.1/2017
  - Supplement 1/2017
- 2016
  - Nr.4/2016
  - Nr.3/2016
  - Nr.2/2016
  - Nr.1/2016
  - Supplement 1/2016
- 2015
  - Nr.4/2015
  - Nr.3/2015
  - Nr.2/2015
  - Nr.1/2015
  - Supplement 1/2015
- 2014
  - Nr.4/2014
  - Nr.3/2014
  - Nr.2/2014
  - Nr.1/2014
  - Supplement 1/2014
- 2013
  - Nr.4/2013
  - Nr.3/2013
  - Nr.2/2013
  - Nr.1/2013
  - Supplement 1/2013
- 2012
  - Nr.4/2012
  - Nr.3/2012
  - Nr.2/2012
  - Nr.1/2012
  - Supplement 1/2012
- 2011
  - Nr.4/2011
  - Nr.3/2011
  - Nr.2/2011
  - Nr.1/2011
  - Supplement 1/2011
- 2010
  - Nr.4/2010
  - Nr.3/2010
  - Nr.2/2010
  - Nr.1/2010
  - Supplement 1/2010
- 2009
  - Nr.4/2009
  - Nr.3/2009
  - Nr.2/2009
  - Nr.1/2009
  - Supplement 1/2009
- 2008
  - Nr.4/2008
  - Nr.3/2008
  - Nr.2/2008
  - Nr.1/2008
  - Supplement 1/2008
- 2007
  - Nr.4/2007
  - Nr.3/2007
  - Nr.2/2007
  - Nr.1/2007
- 2006
  - Nr.4/2006
  - Nr.3/2006
  - Nr.2/2006
  - Nr.1/2006
- 2005
  - Nr.1/2005
Instructions for authors
Online applications
- Send an article
- Online payments
Editorial
About us
Links

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2020): 75%

Journal Metrics

Impact Factor 0.5
Five Year Impact Factor 0.5
JCI 0.12

Top 10 downloaded articles - July 2025 -

ATM rs189037 polymorphism: A p...	18
Romanian Review of Laboratory ...	10
Value of serum NT-IGFBP-4 for ...	9
Investigation of cytokine chan...	8
Association of neutrophil-to-l...	8
Beyond PSA: a multi-modal pilo...	7
Expressions of insulin-like gr...	7
Pilot study on laboratory samp...	7
Immunometabolic profiling of h...	7
Agreement between different eG...	6

Concept, Design & Programming
Dr. Adrian Man

Nr. 15(2)/2009

Development of the acid elution method (Kleihauer test) in the assessment of fetomaternal hemorrhage

Dana Paula Florea, Vladimir Bacârea

Abstract:

Fetomaternal hemorrhage (FMH) is the physiopathological mechanism involved in the hemolytic disease of the newborn (HDN). Its assessment is necessary in order to adjust the dose of anti-D immunoglobulin administered to Rh-negative pregnant women with incompatible pregnancy. Since no assessment test is currently employed in Romania, we propose the acid elution test. Material and method. The paper presents the stages involved in this method’s implementation. After establishing the method and the research batches, the test was performed on samples from healthy adults and Rh D positive newborns. An ANOVA variance test was subsequently performed. Results. The percent of fetal erythrocytes in negative controls allows the use of this sample in order to apply the proposed method. The statistically insignificant difference in variance between the samples in the positive control batch shows variance equivalence between the numbers of detected fetal erythrocytes. Conclusion. The analyzed batches allow initiating the procedures in order to apply the proposed method and assessing its parameters (sensitivity, specificity).

Keywords: hemolytic disease of the newborn,fetomaternal hemorrhage,acid elution,F hemoglobin

Download full text PDF
(197 KB)

Florea DP, Bacârea V. Development of the acid elution method (Kleihauer test) in the assessment of fetomaternal hemorrhage. Rev Romana Med Lab. 2009;15(2):33-9