RRML - Saliva leukocytes rather than saliva epithelial cells represent the main source of DNA

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2020): 75%

Română English

Journal Metrics

Impact Factor 0.5
Five Year Impact Factor 0.5
JCI 0.12

Advanced search

Top 10 downloaded articles
- December 2023 -
Romanian Review of Laboratory ... 4
Markers of cognitive impairmen... 3
Retraction of article: Risk fa... 3
Platelets and Infections... 3
The potential of programmed de... 3
Understanding the key differen... 3
The potential value of some ad... 3
Chronic lymphocytic leukemia d... 2
Hepcidin: a crucial peptide fo... 2
Evaluation of Diagnostic Tests... 2

Log in

Concept, Design & Programming
Dr. Adrian Man

Nr. 24(1)/2016 DOI:10.1515/rrlm-2016-0011

Original professional paper

Saliva leukocytes rather than saliva epithelial cells represent the main source of DNA

Corina Maria Cianga, Ion Antohe, Mihaela Zlei, Daniela Constantinescu, Petru Cianga

Correspondence should be addressed to: Petru Cianga


Introduction. Several alternative methods to peripheral blood DNA extraction have been implemented so far. Saliva seems to represent a very advantageous type of sample, easy to harvest and able to generate DNA yields comparable to those extracted from blood mononuclear cells. Material and methods. 8 patients suspected of ankylosing spondylitis, 9 patients with various hematological malignancies, displaying post-chemotherapy leucopenia and 30 healthy volunteers were included in our study. DNA was extracted with various commercially available kits and used for HLA typing either by PCR amplification, or by PCR followed by hybridization. Results. Our data regarding HLA typing support already published results regarding the good DNA quality that allows its use in various molecular biology techniques. However, when attempting to use saliva from immunosuppressed patients for DNA extraction we have generated very low yields, comparable again with the ones obtained from peripheral blood. Flow cytometry and immunocytochemistry investigations confirmed the low number of leukocytes present in the saliva of these patients, while the number of epithelial cells was virtually unchanged. Conclusions. The main source of saliva DNA seems to be represented by leukocytes present in this fluid and not by the epithelial cells. Under these circumstances, for immunosuppressed patients saliva cannot represent an alternative to blood when attempting DNA extraction.

Keywords: Saliva, DNA, leukocytes, epithelial cells

Received: 16.11.2015
Accepted: 16.2.2016
Published: 8.3.2016

  PDF Download full text PDF
(2188 KB)
How to cite
Cianga CM, Antohe I, Zlei M, Constantinescu D, Cianga P. Saliva leukocytes rather than saliva epithelial cells represent the main source of DNA. Rev Romana Med Lab. 2016;24(1):31-43. DOI:10.1515/rrlm-2016-0011