RRML - Protocol and Matters for Consideration for the Treatment of Polymerase Chain Reaction Contamination in Next-Generation Sequencing Laboratories
AMLR

ISSN online: 2284-5623

ISSN-L: 1841-6624

Rejection rate (2020): 75%

Română English


Journal Metrics

Impact Factor 0.5
Five Year Impact Factor 0.5
JCI 0.12


Advanced search


Top 10 downloaded articles
- June 2024 -
 
The evaluation of a diagnostic... 13
A comprehensive review of Prof... 13
Assessment of ocrelizumab impa... 9
Monitoring the sensitivity of ... 8
Challenges in prophylactic the... 8
Diagnostic value of stress myo... 8
Romanian Review of Laboratory ... 7
Effect of Systemic Inflammator... 7
Understanding the pathogenesis... 6
Evaluation of routine laborato... 6

Log in

Concept, Design & Programming
Dr. Adrian Man

   
 
Nr. 30(4)/2022 DOI:10.2478/rrlm-2022-0038
XML
TXT

Original professional paper

Protocol and Matters for Consideration for the Treatment of Polymerase Chain Reaction Contamination in Next-Generation Sequencing Laboratories

Lin Wang, Chen Ma, Bin He, Rong Qiang, Hongsu Zhang

Correspondence should be addressed to: Lin Wang

Abstract:

Objective: The contamination of polymerase chain reaction (PCR) samples in molecular diagnostic laboratories can cause serious consequences. Internal quality control efforts are often inadequate, especially in clinical next-generation sequencing (NGS) laboratories. Methods: In this study, we retrospectively investigated an incidence of PCR contamination and its decontamination process in a clinical laboratory. We performed a series of measures for decontamination. Taqman fluorescence quantification was carried out to determine the presence of contaminating DNA. SYBR-Green PCR was conducted to evaluate the effect of chlorine disinfectant on NGS library preparation. Results: Through a series of elimination measures undertaken over 8 weeks, the decontamination process was verified as reliable. Almost no contamination was detected. Chlorine disinfectant should be forbidden in Illumina NGS laboratories because it may cause the failure of library preparation.Conclusion: Our prevention and decontamination strategies could effectively eliminate PCR amplicons. Chlorine disinfectants should not be used in Illumina NGS laboratories.

Keywords: laboratory contamination; chlorine disinfectants; high-throughput library

Received: 15.6.2022
Accepted: 14.9.2022
Published: 30.9.2022

 
  PDF Download full text PDF
(619 KB)
     
 
How to cite
Wang L, Ma C, He B, Qiang R, Zhang H. Protocol and Matters for Consideration for the Treatment of Polymerase Chain Reaction Contamination in Next-Generation Sequencing Laboratories. Rev Romana Med Lab. 2022;30(4):467-75. DOI:10.2478/rrlm-2022-0038